Abstrack:
Dengue virus (DV) infection is an endemic disease in tropical and subtropical countries especially Indonesia. Secondary infections with different serotype of dengue virus may cause severe clinical manifestations such as dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). Vaccine development is the promising approach for DV infection prevention. Challenge associated with development of tetravalent subunit vaccines led many researches to improve recombinant vaccines for overcoming this disease. Pre membrane and envelope genes (prM/ E) have been reported as an immunogenic antigen. Several studies revealed that prM/E could give higher immune responses than either structural or non structural proteins. Development of subunit vaccines base on Indonesian local circulating DV is challenging. In this study, prM/ E genes of DENV serotype 3 will be cloned into pET-32b and transforms into E. Coli DH5? then expressed in E. Coli BL21. Western Blot method will be used to detect prM/ E protein and predict epitomes to B cell and T cell using B Cell Epitome Prediction Tools and Genetyx.
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